The preparation of 32P-labelled adenosine polyphosphates

A simple method for the preparation of 32-P-labelled adenosine triphosphate of high specific activity.

The content of adenosine polyphosphates in fatty livers.

It has been reported in a previous paper (Dianzani, 1954) that uncoupling of oxidative phosphorylation occurs in fatty livers. Adenosine triphosphatase (ATPase) activity of mitochondria isolated from fatty livers is increased. Conversely, many substances which inhibit oxidative phosphorylation produce fatty infiltration of the liver when administered intraperitoneally to rats or mice (Dianzani ...

The preparation of biotin-labelled hyaluronidase

Centre and purified using the appropriate Synsorbs (Chembiomed Ltd., Alberta, Canada). Monoclonal anti-A was isolated in the M.R.C. Human Biochemical Genetics Unit. In one experiment lactase from two individuals of different blood group (A and 0) was purified by immunoaffinity chromatography as described in Potter et al. (1985). Using the A blood-group specific antibodies and the lectin from He...

Synthesis of [32P]dolichyl phosphate, utilizing a general procedure for [32P]phosphorus oxychloride preparation.

Equilibration of the phosphorus in radioactive phosphoric acid with the phosphorus in phosphorus oxychloride occurs if these compounds are refluxed together for approximately 24 h. This observation led us to develop a method for the preparation of radioactive phosphorus oxychloride on a small scale with high specific radioactivity. The labeled phosphorus oxychloride may be utilized directly in ...

Acyl coenzyme A synthetase from Pseudomonas fragi catalyzes the synthesis of adenosine 5'-polyphosphates and dinucleoside polyphosphates.

Acyl coenzyme A (CoA) synthetase (EC 6.2.1.8) from Pseudomonas fragi catalyzes the synthesis of adenosine 5'-tetraphosphate (p4A) and adenosine 5'-pentaphosphate (p5A) from ATP and tri- or tetrapolyphosphate, respectively. dATP, adenosine-5'-O-[gamma-thiotriphosphate] (ATP gamma S), adenosine(5')tetraphospho(5')adenosine (Ap4A), and adenosine(5')pentaphospho(5')adenosine (Ap5A) are also substra...